Coordinated regulation of gastric chloride secretion with both acid and alkali secretion.
نویسندگان
چکیده
Gastric secretion of hydrochloric acid requires protons and chloride, yet the mechanisms and regulation of gastric chloride secretion remain unclear. We developed an in vivo technique to simultaneously measure acid/base and chloride secretion into the gastric lumen of anesthetized rats. The cannulated stomach lumen was perfused with weakly pH-buffered chloride-free solution containing a chloride-sensitive fluorophore [5 microM N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE)]. Gastric acid and chloride secretion was detected in gastric effluents by 1) flow-through pH electrode and 2) MQAE fluorescence. Gastric effluent was also collected at 1-min intervals for independent determination of chloride amount by chloridometer. In all conditions, both optical and chemical determinations of chloride report similar amounts of secreted chloride. During luminal perfusion with pH 5 solution, net acid and chloride secretion into the lumen was observed. Pentagastrin stimulated both secretions. In contrast, proton pump inhibition (omeprazole) caused alkalinization of the gastric effluent, but chloride secretion was not diminished. During luminal pH 3 perfusion, net alkali secretion was observed, and chloride secretion at luminal pH 3 was greater than pH 5. When tissue is pretreated with omeprazole at luminal pH 3, the addition of prostaglandin E2 synchronously stimulates both alkali and chloride secretion. Results suggest that both acid and alkali secretions are separately coupled with chloride secretion.
منابع مشابه
Accepted version COORDINATED REGULATION OF GASTRIC CHLORIDE SECRETION WITH BOTH ACID AND ALKALI SECRETION
متن کامل
effect of thyroid hormoned on distension-induced gastrik and pepsin secretion in the stomach of rat
thyroid hormoned are known to influence acid and pepsin secretion,though exact mechanism(s) are not fully understood.in this study distention-stimulated acid and pepsin secretionof hypo and hyperthyroid rats were compared with controls.each group consisted of 8 N-mari rats from both sexs,weighing 246.6+_9.2g. hypo and hyper thyroid states were induced by administration of methimazole(500mg/l H...
متن کاملThe Effects of Acute Consumption of Heroin on Basal and Vagal-Stimulated Gastric Acid and Pepsin Secretion in Rat
Background: Opioid peptides and their receptors are present in the majority of body tissues including gastrointestinal tract. Heroin is one of the opioid derivatives that abuse increasingly today. So far, there is no study on the effect of acute heroin administration on gastric acid and pepsin secretion. Objective: To define the effect of the acute heroin consumption on basal and vagal-stimu...
متن کاملEffects of endogenous production and exogenous administration of H2S on gastric acid secretion in rats
Introduction: Recently, hydrogen sulfide has been introduced as the third gas that acts as a transmitter. It has many physiological and pharmacological roles in the human body. The aim of the present study was to investigate the effect of exogenously administered and endogenously produced H2S on the basal and distention-induced gastric acid secretion in rats. Methods: Forty-nine male Wistar...
متن کاملEffect of Esophageal Distention on Basal and Stimulated Gastric Acid Secretion in Rats
It is well established that the esophageal distention (ED) leads to gastric relaxation, partly by vago-vagal reflex, but till now, the effect of ED on gastric acid secretion has not been investigated. The aim of this study was to investigate the effect of ED on basal and stimulated gastric acid secretion. Methods: Adult male Wistar rats (200-240 g) were deprived of food but not the water 24 h b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- American journal of physiology. Gastrointestinal and liver physiology
دوره 283 5 شماره
صفحات -
تاریخ انتشار 2002